NURSING ANM AND GNM

Detailed explanation-1: -The use of a thermophilic DNA polymerase such as Taq polymerase prevents the denaturation of the enzyme during the heating step that is necessary to separate the newly synthesized strand – this subsequently simplifies the PCR technique and increases its efficiency.

Detailed explanation-2: -Taq polymerase Like DNA replication in an organism, PCR requires a DNA polymerase enzyme that makes new strands of DNA, using existing strands as templates. The DNA polymerase typically used in PCR is called Taq polymerase, after the heat-tolerant bacterium from which it was isolated (Thermus aquaticus).

Detailed explanation-3: -The thermostable DNA polymerase is perhaps the most important target site of PCR-inhibiting substances. The most widely used polymerase in PCR-based methods for the detection of microorganisms is Taq DNA polymerase from Thermus aquaticus.