NURSING ANM AND GNM

Detailed explanation-1: -Heterogeneous immunoassays require separation of the analyte‐antibody complex from the remaining sample prior to final analysis. This can be accomplished using precipitating chemicals, cross‐linking with other antibodies or use of an antibody bound to a solid phase.

Detailed explanation-2: -Enzyme-linked immunosorbent assay (ELISA) is a plate-based immunological assay designed to detect and quantify peptides, proteins, antibodies, and hormones. Fluorescence polarization (FP) is a solution-phase technique that can be used to determine equilibrium dissociation constant of ligand for the protein of interest.

Detailed explanation-3: -Fluorescence polarization immunoassay (FPA) is a homogeneous immunoassay useful for rapid and accurate detection of antibody or antigen. The principle of the assay is that a fluorescent dye (attached to an antigen or an antibody fragment) can be excited by plane-polarized light at the appropriate wavelength.

Detailed explanation-4: -Fluorescent excitation transfer immunoassay. Fluorescence polarization immunoassay (FPIA) Time-Resolved Fluorescence Immunoassays (TRFIA) Fluorescence Energy Transfer Immunoassays. Phase-Modulation Fluorescence Spectroscopy. Phase-Resolved Fluoroimmunoassays. More items