NURSING ANM AND GNM

Detailed explanation-1: -Due to the difference in density of the plasma and the red and white blood cells, blood cells are driven toward the bottom while the plasma forms the supernatant layer on the top [101] .

Detailed explanation-2: -In the second phase of the procedure, the tube is centrifuged so that its contents separate into three layers-packed red blood cells (erythrocytes) at the bottom, a reddish gray layer of white blood cells (leukocytes) and platelets in the middle, and plasma at the top.

Detailed explanation-3: -A machine called a centrifuge spins your blood to separate your red blood cells, platelets and plasma. As the blood is separated, the heavier reds cells sink to the bottom and are given back to you.

Detailed explanation-4: -After centrifugation, the sample is separated into four distinct layers. From the bottom to the top of the aspirate in the Wintrobe tube (shown in the image on the right) are erythrocytes (E)/red blood cells (RBCs), myeloid-erythroid (M-E) cells (buffy coat), plasma (P), and the fat and perivascular (F-PV) cells.