NURSING EXAM QUESTIONS
TOXICOLOGY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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spectrometer, spectrophotometer
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spectrophotometer, spectrometer
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Either A or B
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None of the above
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Detailed explanation-1: -Spectrophotometry is a method to measure how much a substance absorbs light by measuring the intensity of light as a beam of light passes through sample solution. The basic principle is that each compound absorbs or transmits light over a certain range of wavelength.
Detailed explanation-2: -What is absorbance measured in? Absorbance is measured using a spectrophotometer or microplate reader, which is an instrument that shines light of a specified wavelength through a sample and measures the amount of light that the sample absorbs.
Detailed explanation-3: -Absorbance Wavelengths A UV-visible spectrophotometer uses light over the ultraviolet range (185 – 400 nm) and visible range (400 – 700 nm) of the electromagnetic radiation spectrum. Whereas an IR spectrophotometer uses light over the infrared range (700 – 15000 nm).