NURSING ANM AND GNM

Detailed explanation-1: -Biuret solution is made by mixing copper sulfate with sodium hydroxide. Copper sulfate gives a typical blue color to this solution, which is turned to purple after reacting with the proteins.

Detailed explanation-2: -What is the Biuret Test? The Biuret test is used to detect the presence of proteins in a sample. The test is founded on the idea that copper ions in an alkaline solution can form a complex with the peptide bonds in proteins, resulting in a change in color, from blue to violet.

Detailed explanation-3: -The biuret reaction is based on the complex formation of cupric ions with proteins. In this reaction, copper sulfate is added to a protein solution in strong alkaline solution. A purplish-violet color is produced, resulting from complex formation between the cupric ions and the peptide bond.

Detailed explanation-4: -Biuret reagent is an alkaline solution of 1% CuSO4, copper sulfate. The violet color is a positive test for the presence of protein, and the intensity of the color is proportional to the number of peptide bonds in the solution.