PATHOLOGY

Detailed explanation-1: -Metaphase. Chromosomes line up at the metaphase plate, under tension from the mitotic spindle. The two sister chromatids of each chromosome are captured by microtubules from opposite spindle poles. In metaphase, the spindle has captured all the chromosomes and lined them up at the middle of the cell, ready to divide.

Detailed explanation-2: -Metaphase. During metaphase, spindle fibers attach to the centromere of each pair of sister chromatids (see Figure below). The sister chromatids line up at the equator, or center, of the cell. This is also known as the metaphase plate.

Detailed explanation-3: -Long protein fibers called microtubules extend from the centrioles in all possible directions, forming what is called a spindle. Some of the microtubules attach the poles to the chromosomes by connecting to protein complexes called kinetochores.

Detailed explanation-4: -The spindle fibers form during prophase. During metaphase of cell division, the spindle fibers radiate from the centrioles at the opposite poles. Some of them attach to the kinetochores of the chromosomes while others bind to the arms of the chromosomes, still others continue to grow.