PATHOLOGY MCQ
GENETICS AND DISEASE
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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PCR
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Karyotyping
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Electrophoresis
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Cloning
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Detailed explanation-1: -After the DNA has been digested with one or more restriction enzymes, it is separated by size using gel electrophoresis. A radioactive probe is then used to identify certain fragments of DNA that may differ between individuals.
Detailed explanation-2: -Gel electrophoresis is a technique used to separate DNA fragments according to their size. DNA samples are loaded into wells (indentations) at one end of a gel, and an electric current is applied to pull them through the gel. DNA fragments are negatively charged, so they move towards the positive electrode.
Detailed explanation-3: -The DNA fingerprinting technique involves the DNA extracted and sequenced with the help of gel electrophoresis to create a genetic fingerprint. The DNA samples taken from the crime scenes are compared, and if the DNA profile matches, the suspect is determined.