PATHOLOGY MCQ
GENETICS AND DISEASE
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Gel Electrophoresis
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PCR
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Restriction enzymes
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This isn’t possible
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Detailed explanation-1: -PCR is used in molecular biology to make many copies of (amplify) small sections of DNA? or a gene?. Using PCR it is possible to generate thousands to millions of copies of a particular section of DNA from a very small amount of DNA. PCR is a common tool used in medical and biological research labs.
Detailed explanation-2: -Sometimes called “molecular photocopying, ‘’ the polymerase chain reaction (PCR) is a fast and inexpensive technique used to “amplify”-copy-small segments of DNA.
Detailed explanation-3: -A thermal cycler (also known as a PCR machine or thermocycler) is a laboratory instrument that heats and cools samples in repetitive cycles to facilitate DNA or RNA amplification through the polymerase chain reaction.
Detailed explanation-4: -So, the correct answer is ‘Thermus aquaticus’
Detailed explanation-5: -Throughout the PCR process, DNA is subjected to repeated heating and cooling cycles during which important chemical reactions occur. During these thermal cycles, DNA primers bind to the target DNA sequence, enabling DNA polymerases to assemble copies of the target sequence in large quantities.