PATHOLOGY MCQ
GENETICS AND DISEASE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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By activating or deactivating specific genes
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By calculating the number of genes different organisms produce
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By identifying how closely related one individual is to another
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By determining the number of chromosomes in an organism
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Detailed explanation-1: -CRISPR/Cas9 edits genes by precisely cutting DNA and then letting natural DNA repair processes to take over. The system consists of two parts: the Cas9 enzyme and a guide RNA. Rapidly translating a revolutionary technology into transformative therapies.
Detailed explanation-2: -CRISPR/Cas9 works by cutting a DNA sequence at a specific genetic location and deleting or inserting DNA sequences, which can change a single base pair of DNA, large pieces of chromosomes, or regulation of gene expression levels.
Detailed explanation-3: -CRISPR–Cas9 is a unique technology that enables geneticists and medical researchers to edit parts of the genome by removing, adding or altering sections of the DNA sequence. It is currently the simplest, most versatile and precise method of genetic manipulation and is therefore causing a buzz in the medical field.
Detailed explanation-4: -Long story short: 1) Decide which gene you want to cut. 2) Design a gRNA to target a specific PAM sequence near that region. 3) Express that gRNA in the cell of interest in addition to an endonuclease protein such as Cas9 or Cpf1. 4) Voila!