PATHOLOGY MCQ
GENETICS AND DISEASE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To produce millions of copies of DNA.
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To produce millions of copies of a specific region of DNA
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To add nucleotides to a DNA sequence
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To watch polymerase work.
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Detailed explanation-1: -PCR is a very sensitive technique that allows rapid amplification of a specific segment of DNA. PCR makes billions of copies of a specific DNA fragment or gene, which allows detection and identification of gene sequences using visual techniques based on size and charge.
Detailed explanation-2: -PCR is used in molecular biology to make many copies of (amplify) small sections of DNA? or a gene?. Using PCR it is possible to generate thousands to millions of copies of a particular section of DNA from a very small amount of DNA. PCR is a common tool used in medical and biological research labs.
Detailed explanation-3: -Why does PCR amplify only one specific region of DNA rather than all of it? The primers mark the ends, ensuring that only the DNA within the region between is amplified.
Detailed explanation-4: -Polymerase chain reaction, or PCR, is a laboratory technique used to make multiple copies of a segment of DNA.
Detailed explanation-5: -Polymerase chain reaction (abbreviated PCR) is a laboratory technique for rapidly producing (amplifying) millions to billions of copies of a specific segment of DNA, which can then be studied in greater detail.