PATHOLOGY MCQ
GENETICS AND DISEASE
|
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
|
|
|
The process of cutting DNA with restriction enzymes.
|
|
|
The process of the gene of interest being put in the vector.
|
|
|
The process of the gene of interest being taken into the host cell’s DNA & being expressed on the outside of the cell.
|
|
|
The process selecting a gene of interest to cut with restriction enzymes.
|
Detailed explanation-1: -Bacteria can take up foreign DNA in a process called transformation. Transformation is a key step in DNA cloning . It occurs after restriction digest and ligation and transfers newly made plasmids to bacteria. After transformation, bacteria are selected on antibiotic plates.
Detailed explanation-2: -Plasmid or vector transformation is the process by which exogenous DNA is transferred into the host cell. Transformation usually implies uptake of DNA into bacterial, yeast or plant cells, while transfection is a term usually reserved for mammalian cells.
Detailed explanation-3: -Transformation is one mode of horizontal gene transfer (HGT) in bacteria, wherein extracellular naked DNA is taken up by cells that have developed genetic competence. Sensitivity to DNase, which degrades naked DNA, is the key to distinguishing transformation from the DNase-resistant HGT mechanisms.
Detailed explanation-4: -A vector, as related to molecular biology, is a DNA molecule (often plasmid or virus) that is used as a vehicle to carry a particular DNA segment into a host cell as part of a cloning or recombinant DNA technique.