PATHOLOGY MCQ
GENETICS AND DISEASE
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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a gene that increases the cell’s resistance to diseases
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a gene that increased the range of temperatures in which the cell could survive
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a gene that reduced the amount of variation in the offspring of the host organism
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a gene that reduced the cell’s energy requirements
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Detailed explanation-1: -The simplest way of gene introduction is an injection of naked DNA in to target cells. The use of non-viral vectors can be in the form of injections of naked DNA (usually plasmids), liposomes or particle-mediated gene transfer (’the gene gun’).
Detailed explanation-2: -For gene-transfer experiments, the preferred route is the culture of protoplasts-single cells from which the cellulose wall has been removed. That is because it is easier to insert genes into protoplasts than into cells containing the tough outer wall, which animal cells lack.
Detailed explanation-3: -There are three mechanisms of horizontal gene transfer in bacteria: transformation, transduction, and conjugation. The most common mechanism for horizontal gene transmission among bacteria, especially from a donor bacterial species to different recipient species, is conjugation.
Detailed explanation-4: -A vector is a molecule of DNA that is attached to a foreign gene to facilitate its transfer, maintenance, and expression within the target cell.