PATHOLOGY MCQ
INFLAMMATION
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Flow chart of the peptide screening of the random peptide library
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Flowchart of the peptide screen of the library of correct-constructs
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Either A or B
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None of the above
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Detailed explanation-1: -For phage display, the antigen-binding regions of VH and VL genes are cloned and used to construct scFv (or Fab) gene repertoires. A phage antibody library is created by cloning these repertoires as fusion proteins with a minor coat protein of bacteriophage (the gene 3 protein) (10–12).
Detailed explanation-2: -Phage display is a potent and versatile method for studying protein-protein interaction. It can be applied to a wide range of protein interaction partners and used in a number of applications, especially in mapping intracellular interactions of the distinct protein domain.
Detailed explanation-3: -Phage display is a laboratory technique for the study of protein–protein, protein–peptide, and protein–DNA interactions that uses bacteriophages (viruses that infect bacteria) to connect proteins with the genetic information that encodes them.
Detailed explanation-4: -Libraries of phage-displayed peptides or proteins are thereby physically linked to their encoding nucleic acid, allowing selection of binding partners for myriad target types by iterative rounds of in vitro panning and amplification, followed by DNA sequencing.