PATHOLOGY

Detailed explanation-1: -When properly applied, this technique-known as the chromium hematoxylin-phloxine method-stained the islet B cells an intensely deep blue and the A cells bright red. The D cells stained capriciously and variably pink-to-red in hue and were difficult to distinguish from islet A cells on the basis of color.

Detailed explanation-2: -Azocarmine is frequently used as a counter-stain to color the cells and collagenous tissue red. (Golgi): A black deposit of reduced silver is laid down on the surfaces of nerve cells and neuroglia cells so that the form of the cell body and its processes stand out prominently in an almost colorless background.