GENERAL KNOWLEDGE

Detailed explanation-1: -Four separation techniques based on molecular characteristics and interaction type use mechanisms of ion exchange, surface adsorption, partition, and size exclusion. Other chromatography techniques are based on the stationary bed, including column, thin layer, and paper chromatography.

Detailed explanation-2: -chromatography, technique for separating the components, or solutes, of a mixture on the basis of the relative amounts of each solute distributed between a moving fluid stream, called the mobile phase, and a contiguous stationary phase.

Detailed explanation-3: -Elution chromatography is the most wide-spread method used today. The sample components, which adsorb to a stationary phase, will be eluted by an increasing concentration gradient of a solvent component with a high elution power. The order of elution corresponds to the affinity of each sample component to the column.

Detailed explanation-4: -Chromatography is a method by which a mixture is separated by distributing its components between two phases. The stationary phase remains fixed in place while the mobile phase carries the components of the mixture through the medium being used.