HORTICULTURE

Detailed explanation-1: -It should have a selectable marker with an antibiotic resistance gene that facilitates screening of the recombinant organism. It should be small in size so that it can easily integrate into the host cell. It should be capable of inserting a large segment of DNA.

Detailed explanation-2: -If a cloned vector does not have a selectable marker, it will be impossible to distinguish between transformants (host bacteria with rDNA) and non-transformants. Therefore, an ideal cloning vector should have selectable markers for the selection of transformants.

Detailed explanation-3: -Due to the presence of the selective marker, the plasmid becomes useful for the cell. Under the selective conditions, only cells that contain plasmids with the appropriate selectable marker can survive.

Detailed explanation-4: -Selectable markers are genes that help identify bacteria that have successfully transformed, or taken up the recombinant plasmid. Antibiotic resistance genes are commonly used markers.