MICROANATOMY

Detailed explanation-1: -Centrifuge WB at a ‘high’ speed. Three layers are formed because of its density: The bottom layer consisting of RBCs, the middle layer consisting of platelets and WBCs and the top PPP layer.

Detailed explanation-2: -Both WBCs and platelets are present and prevalent in the buffy coat.

Detailed explanation-3: -A buffy coat is a concentration of white blood cells (WBCs) and platelets. WBCs and platelets make up only around 1% of the cells in the volume of peripheral whole blood. When a blood sample is centrifuged, the platelets and WBCs unite to form a layer floating between the RBCs and supernatant plasma.

Detailed explanation-4: -The term “buffy coat” might make you think of a shiny car wax, but in the world of blood banking, buffy coat refers to the white layer between red blood cells and plasma in a unit of whole blood after it has been spun down in a centrifuge. The buffy coat contains white blood cells, the soldiers of the immune system.

Detailed explanation-5: -Following centrifugation, the blood components are separated into distinct layers based on their density. The white blood cell (WBC) rich buffy coat can be found at the erythrocytes-plasma junction, composing <1% of the total blood volume.