MICROANATOMY

Detailed explanation-1: -The light yellow colored liquid on the top is the plasma, which accounts for about 55 percent of the blood volume and red blood cells is called the hematocrit, or packed cell volume (PCV). The white blood cells and platelets form a thin white layer, called the “buffy coat", between plasma and red blood cells.

Detailed explanation-2: -What is the Buffy Coat? The buffy coat accounts for less than 1 percent of a whole blood sample, yet it contains the majority of white blood cells (WBC) and platelets. In fact, leukocytes can be 10-20X more concentrated in the buffy coat than in whole blood.

Detailed explanation-3: -A buffy coat contains leukocytes in a concentrated suspension, originating from whole blood or bone marrow. Generating a buffy coat from whole blood samples helps to concentrate large sample volumes and reduce downstream cell separation handling.

Detailed explanation-4: -The buffy coat is simply a concentration of all the white blood cells and platelets in a sample of blood.

Detailed explanation-5: -Composing less than 1% of the total volume of the blood sample, the buffy coat (so-called because it is usually buff in hue), contains most of the white blood cells and platelets.