MICROANATOMY

Detailed explanation-1: -Luminol produces a bluish-white light when peroxidase is added to it in the presence of blood and it is the heme portion of hemoglobin that reacts with the peroxidase. The color and intensity of the light can be measured. Samples of bone from 80 nonpathological femurs were subjected to luminol testing.

Detailed explanation-2: -In reacting with an oxidizing agent, Luminol generates a blue chemiluminescence, especially in the presence of catalytic elements. In case of the detection of blood, the Luminol solution, which contains hydrogen peroxide, is applied to blood.

Detailed explanation-3: -Scientists can take advantage of the luminol reaction to locate potential blood evidence that would be undetectable through visual examination.

Detailed explanation-4: -In the demonstration vial is a mix of luminol, perborate and copper sulphate. When water is added the copper sulphate dissolves and reacts with the luminol. This forms a molecule that has an excited electronic state. The molecule then sheds this extra energy in the form of light.

Detailed explanation-5: -Luminol is a common method for preliminary identification of blood. While other types of presumptive tests for the identification of blood exist, luminol remains as a frequently used method world-wide. This is partially due to its ability to be used to screen large areas for the presence of blood.