MICROANATOMY

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INTRODUCTION TO MICROSCOPY CELL BIOLOGY

DIFFERENTIATED CELL

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
In a famous experiment in 1962, John Gurdon successfully cloned a frog using a nucleus from a body cell of a tadpole. Gurdon’s experiment captured the attention of the scientific community. The tools and techniques he developed for nuclear transfer are still used today. He destroyed the DNA of a fertilised frog egg with UV light and transplanted into it a nucleus from an intestinal cell of a tadpole. Some of the surviving eggs developed into normal tadpoles and normal frogs. This experiment demonstrates that:
A
differentiation does not destroy the genetic information in cells.
B
adult frogs can be developed from haploid eggs.
C
protein synthesis ceases after DNA is destroyed by UV light.
D
frog intestinal cells and unfertilised eggs contain the same amount of DNA.
Explanation: 

Detailed explanation-1: -Gurdon’s research built on the work of Thomas King and Robert Briggs in the United States, who in 1952 published findings that indicated that scientists could take a nucleus from an early embryonic cell and successfully transfer it into an unfertilized and enucleated egg cell.

Detailed explanation-2: -However, in the 1950s American scientists Robert Briggs and Thomas King successfully cloned tadpoles using nuclear transfer of frog blastula cells, which have lost some of their totipotent properties.

Detailed explanation-3: -Briggs and King were the first to perform cloning by nuclear transfer using eggs and cells from the Northern Leopard Frog, Rana pipiens. Although these experiments were successful when Briggs and King used unspecialised cells, they found that they could not make cloned frogs when they used more specialised cells.

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