INTRODUCTION TO MICROSCOPY CELL BIOLOGY
LIGHT MICROSCOPY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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True
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False
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Sometimes
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None of the above
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Detailed explanation-1: -Resolution is also related to the wavelength of light which is used to image a specimen; light of shorter wavelengths are capable of resolving greater detail than longer wavelengths.
Detailed explanation-2: -So, if the wavelength of a light wave is shorter, that means that the frequency will be higher because one cycle can pass in a shorter amount of time. This means that more cycles can pass by the set point in 1 second.
Detailed explanation-3: -Generally short wavelengths mean smaller and less expensive equipment. Short wavelength radars are more effective in detecting small particles such as cloud droplets and drizzle drops. However, the short wavelength electromagnetic energy is also partially absorbed by these same particles (a process called attenuation).
Detailed explanation-4: -IUPAC definition IUPAC defines resolution in optical spectroscopy as the minimum wavenumber, wavelength or frequency difference between two lines in a spectrum that can be distinguished. Resolving power, R, is given by the transition wavenumber, wavelength or frequency, divided by the resolution.