MICROANATOMY

Detailed explanation-1: -Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations.

Detailed explanation-2: -A phase plate is mounted in or near the objective rear focal plane (see Figures 4 and 5) in order to selectively alter the phase and amplitude of the surround (or undeviated) light passing through the specimen.

Detailed explanation-3: -Phase contrast is an optical contrast technique for microscopy which makes unstained structures in the cells of biological specimens visible. Cell structures that appear transparent with brightfield illumination can be viewed in high contrast and rich detail using phase contrast.