MICROANATOMY

INTRODUCTION TO MICROSCOPY CELL BIOLOGY

LIGHT MICROSCOPY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
What is the maximum resolution of light microscopes?
A
100 nm
B
200 nm
C
300 nm
D
400 nm
Explanation: 

Detailed explanation-1: -The best resolution for an optical microscope is about 0.2 microns = 200 nm.

Detailed explanation-2: -This means that we will never be able to see any object smaller than approximately 200 nm (about the width of an average-sized bacterium) using a light microscope (and in practice, many light microscopes can’t get close to this resolution because of lens quality).

Detailed explanation-3: -Conventional light microscopes have a resolution limit of 200 nanometers, meaning that any objects closer than this distance will not be observed as separate objects.

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