INTRODUCTION TO MICROSCOPY CELL BIOLOGY
LIGHT MICROSCOPY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What is the maximum resolution of light microscopes?
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100 nm
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200 nm
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300 nm
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400 nm
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Explanation:
Detailed explanation-1: -The best resolution for an optical microscope is about 0.2 microns = 200 nm.
Detailed explanation-2: -This means that we will never be able to see any object smaller than approximately 200 nm (about the width of an average-sized bacterium) using a light microscope (and in practice, many light microscopes can’t get close to this resolution because of lens quality).
Detailed explanation-3: -Conventional light microscopes have a resolution limit of 200 nanometers, meaning that any objects closer than this distance will not be observed as separate objects.
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