MICROANATOMY

Detailed explanation-1: -The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 µm), the objects which are, at most, about 0.2 µm.

Detailed explanation-2: -Answer and Explanation: The resolution of a light microscope cannot be smaller than half of the wavelength of the visible light ( = 400-700 nm).

Detailed explanation-3: -Wavelength is the main limiting factor on resolution because the image of two particles cannot be seen individually if it is smaller than the wavelength.