INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To make sure you don’t crush your specimen
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To avoid air bubbles on the slide
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To give the specimen time to get comfortable
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To make sure you don’t break the slide
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Detailed explanation-1: -Slowly and gently lower the coverslip onto the slide so it covers the sample. In using this technique, not only do you reduce the possibility of damaging the tissue, but you reduce the air bubbles that might occur underneath the coverslip.
Detailed explanation-2: -Applying the cover slip at an angle (instead of dropping it down flat on the specimen) pushes the air to the side and therefore minimizes the risk of air bubbles.
Detailed explanation-3: -Place the coverslip at a 45-degree angle with one edge touching the water and let go. The coverslip will fall over the sample and be held in place by surface tension. (This method should prevent any air bubbles under the coverslip, as these will affect your ability to view the sample.)