MICROANATOMY

Detailed explanation-1: -Close the eye you just used and look through the other eyepiece with the other eye. Don’t touch the focus knobs, but rather adjust the diopter on the eyepiece if the image is out of focus at all. Move up to the highest magnification objective. Repeat the procedure using the highest objective lens.

Detailed explanation-2: -While looking down the eyepiece of the microscope, you can then turn the coarse-focus knob until the contents of the slide become clearer and more distinct. After this, you can use the smaller, fine-focus knob to make the specimen as clear as possible.

Detailed explanation-3: -This is a particular problem for the higher magnifications, where the focus can be lost much more easily. If this happens, then you are much faster to simply start over again. Rotate the low magnification objective into place again and re-focus and re-center the image before moving to the higher magnifications.