INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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DAPI, nucleus, blue
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DAPI, actin filaments, green
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A mushroom toxin, nucleus, blue
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A mushroom toxin, actin filaments, red
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A mushroom toxin, nucleus, red
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Detailed explanation-1: -Phalloidin binds F-actin with high selectivity while Alexa Fluorâ„¢ 555 provides red-orange fluorescence of unparalleled brightness and photostability. Demonstrating very little nonspecific staining, Alexa Fluorâ„¢ 555 phalloidin allows high-contrast discrimination of actin staining.
Detailed explanation-2: -Why do we use stains to identify the location of cellular structures? Makes structures easier to see under microscope (better contrast). Which stain will you use to identify the location of cell nuclei? Some stains bind to specific molecules within cells and emit light when viewed using a fluorescence microscope.
Detailed explanation-3: -Phalloidin is a highly selective bicyclic peptide used for staining actin filaments (also known as F-actin). It binds to all variants of actin filaments in many different species of animals and plants.