MICROANATOMY

Detailed explanation-1: -Limitations of optical microscopy The resolution of the optical microscope is limited to 0.2 micrometers and the practical magnification limit is 1000x.

Detailed explanation-2: -The resolution of the light microscope cannot be small than the half of the wavelength of the visible light, which is 0.4-0.7 µm. When we can see green light (0.5 µm), the objects which are, at most, about 0.2 µm. Below this point, light microscope is not useful, as wavelength smaller than 400 nm is needed.

Detailed explanation-3: -Therefore the electron microscope has a maximum focus of 0.2 nanometers. This is equivalent to the distance between two atoms in a solid and 1000 times greater than that of a light microscope.