MICROANATOMY

Detailed explanation-1: -The final step in gram staining is to use basic fuchsin stain to give decolorized gram-negative bacteria pink color for easier identification. It is also known as counterstain. Some laboratories use safranin as a counterstain; however, basic fuchsin stains gram-negative organisms more intensely than safranin.

Detailed explanation-2: -The reagent that is used as a counter stain in gram staining is called safranin. This stain is pink and is added at the last step of the gram-staining process after decolorization with alcohol has taken place.

Detailed explanation-3: -For instance, in Gram staining, two dyes are used: the Crystal Violet and Safranin. The Safranin is the counterstain used in this method. The Gram Staining is used to distinguish Gram positive bacteria from Gram negative bacteria.