INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Eyepiece graticules and stage micrometers are used to measure cells. Which is the correct reason why an eyepiece graticule is calibrated?
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An eyepiece graticule can be used to make measurement
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An eyepeice graticule is magnified by the objective lens
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An eyepiece graticule magnifies the specimen
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An eyepiece graticule makes comparison
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Explanation:
Detailed explanation-1: -So you need to calibrate your eyepiece graticule with a stage micrometer before making measurements with your graticule. The graticule scale is arbitrary – it represents different lengths for different magnifications, so the scale needs to be calibrated for any new microscope or new magnification.
Detailed explanation-2: -One division on the eyepiece graticule is equivalent to 250 51 m on the stage micrometer: = 4.9 m (to two significant figures ). Therefore one division is equal to 4.9 m. Using the same calibrated eyepiece graticule to measure a cell: The width of the cell highlighted = 52 – 40 = 12 eyepiece graticule divisions.
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