MICROANATOMY

Detailed explanation-1: -Fixation of the sample has to occur first so that the sample appears as close as possible to a living sample with no loss of proteins, lipids or other molecules. Cross-linking fixation is a technique in which aldehyde groups cross link molecules within the cell, though this can hinder antigen binding.

Detailed explanation-2: -Why do we use stains to identify the location of cellular structures? Makes structures easier to see under microscope (better contrast). Which stain will you use to identify the location of cell nuclei? Some stains bind to specific molecules within cells and emit light when viewed using a fluorescence microscope.