INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Second outer membrane that helps to retain the crystal violet stain
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Multiple layer of peptidoglycan that helps to retain the crystal violet stain
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Thick capsule that traps the crystal violet stain
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Periplasmic space that traps the crystal violet
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Detailed explanation-1: -The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with.
Detailed explanation-2: -Peptidoglycan provides rigidity to the cell wall; the cell walls of Gram-positive bacteria may contain up to 40 layers of peptidoglycan, conferring significant mechanical strength.
Detailed explanation-3: -Peptidoglycan is a component of the bacterial cell wall which gives structural strength to the cell. A thick layer of peptidoglycan retains the primary stain (crystal violet) so Gram-positive cells are stained purple.