MICROANATOMY

INTRODUCTION TO MICROSCOPY CELL BIOLOGY

MICROSCOPY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
How is the image built by the objective lens when observing a sample?
A
By focusing the excitation beam to a certain points of the sample
B
By passing the excitation beam towards the sample
C
By splitting the beam from the light source into two separate beams
D
None of the above
Explanation: 

Detailed explanation-1: -In a compound microscope the image produced by the objective is real, enlarged and inverted because objective has smaller focal length than the object distance so, image is formed which is real, magnified and inverted.

Detailed explanation-2: -How to Focus Your Microscope: The proper way to focus a microscope is to start with the lowest power objective lens first and while looking from the side, crank the lens down as close to the specimen as possible without touching it. Now, look through the eyepiece lens and focus upward only until the image is sharp.

Detailed explanation-3: -Start by rotating the objective lens to lowest power. Place a slide on the stage, label side up, with the coverslip centered. On LOW POWER ONLY, use the coarse focus knob to get the object into focus. If you cannot see anything, move the slide slightly while viewing and focusing. More items

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