MICROANATOMY

Detailed explanation-1: -Immunofluorescence microscopy is a powerful technique that is widely used by researchers to assess both the localization and endogenous expression levels of their favorite proteins.

Detailed explanation-2: -Primary, or direct, immunofluorescence uses a single antibody that is conjugated directly to a fluorescent dye. The antibody recognizes the target molecule, binds to it, and the conjugated fluorescent dye can be detected using a microscope.

Detailed explanation-3: -The simplest microscope design used for examples of immunofluorescence samples is the epifluorescence microscope. Another common design is the confocal microscope. Also commonly used are various designs of super-resolution microscopes which are capable of much higher resolution levels.