INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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In negative stains, we rely on the ____ charge of the cell structure to repel the stain.
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Positive
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Negative
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Either A or B
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None of the above
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Explanation:
Detailed explanation-1: -Negative staining requires the use of acid stain such as the Indian ink or nigrosin. The acid stain with its negatively charged chromogen will not penetrate the cells because of the negative charge on the surface of the bacteria.
Detailed explanation-2: -In contrast to direct stains that bind to bacteria directly, a negative stain colors the background of a smear rather than the bacteria. These stains have negatively charged functional groups so they can’t bind directly to negatively charged bacteria.
There is 1 question to complete.