INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The location of which structure will you see in the cell using DAPI?
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Actin Protein
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Tubulin protein
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Nucleus
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Microtubules
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Explanation:
Detailed explanation-1: -It is believed that DAPI associates with the minor groove of double-stranded DNA, with a preference for the adenine-thymine clusters.
Detailed explanation-2: -DAPI (4’, 6-diamidino-2-phenylindole) (Figure 1) is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. DAPI permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei.
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