INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Spinning Disk Confocal Microscopes
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Hybrid Scanning Confocal Microscopes
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Liquid Scanning Confocal Microscopes
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Laser Scanning Confocal Microscopes
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Detailed explanation-1: -Confocal microscopy is widely used for fluorescence imaging in the life sciences. The last decade has seen advances in illumination sources, detectors, fluorescent probes, optics, and sample preparation techniques, which provide improvements in different combinations of speed, depth, and resolution.
Detailed explanation-2: -Mercury and Xenon Light sources are to week for point confocal systems. Strong bundled light is generated by LASERS. There are different types of LASERS: Argon, Argon-Krypton, Helium-Neon, etc.
Detailed explanation-3: -Confocal microscopy uses light from a laser through the objective of a standard light microscope to excite a specimen within a narrow plane of focus. Any emission of light from out-of-focus planes is rejected by the pinhole, or confocal aperture.