INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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What are the principles behind microscope?
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Illumination
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Magnification
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Focusing
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Numerical Aperture
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Resolution
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Explanation:
Detailed explanation-1: -The resolution of a microscope is determined by both the wavelength of light () and the maximum angle of light that can be collected by the objective lens. For a sample mounted on a slide and viewed with an objective lens designed to observe a specimen through an air medium (n = 1.0), the N.A. is simply sin().
Detailed explanation-2: -Key Points Magnification is the ability to make small objects seem larger, such as making a microscopic organism visible. Resolution is the ability to distinguish two objects from each other. Light microscopy has limits to both its resolution and its magnification.
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