MICROANATOMY

Detailed explanation-1: -In the direct-method, the fluorochrome-labeled antibodies are used as probes. The indirect method, on the other hand, uses non-labeled antibodies for the particular biomolecules as the primary antibodies. The fluorochrome-labeled secondary antibodies are used for fluorescent labeling of the primary antibodies.

Detailed explanation-2: -Ethidium bromide, fluorescein and green fluorescent protein are common tags. The most commonly labelled molecules are antibodies, proteins, amino acids and peptides which are then used as specific probes for detection of a particular target.

Detailed explanation-3: -Protein labeling with fluorescent probes can be accomplished by linking cyanine dyes, rhodamine dyes, or fluorescein to cysteines, lysines, tyrosines, or the N-terminus of your target protein.

Detailed explanation-4: -Immunofluorescence assays use antibody-fluorogen conjugates to illuminate antigens for easy, rapid detection. Direct immunofluorescence can be used to detect the presence of bacteria in clinical samples such as sputum.