MICROANATOMY

Detailed explanation-1: -Confocal laser scanning microscope consists of a pinhole mechanism which selectively filters in the fluorescence signals from the region of the sample where the laser is focused. This fluorescence generated from the regions surrounding the focal point is blocked by the pinhole mechanism.

Detailed explanation-2: -A pinhole camera is a simple camera without a lens but with a tiny aperture (the so-called pinhole)-effectively a light-proof box with a small hole in one side. Light from a scene passes through the aperture and projects an inverted image on the opposite side of the box, which is known as the camera obscura effect.

Detailed explanation-3: -Confocal microscopy is a narrow-field imaging technique where an excitation laser source is focused to a single point using a pinhole to cut out of focus light.

Detailed explanation-4: -The confocal microscope essentially removes the out-of-focus light by inserting a pinhole at the image plane such that out-of-focus light does not reach the detector (Figure 2B). Only light focused at the pinhole passes through it, all other light is scattered.