INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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To distinguish between different structure
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To maintain the structure of the cell
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To remove plasma membrane constituents
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None of the above
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Detailed explanation-1: -Fixation. Fixation is a crucial step in preparing specimens for microscopic examination. Its objective is to prevent decay and preserve cells and tissues in a “life-like” state.
Detailed explanation-2: -The broad objective of tissue fixation is to preserve cells and tissue components in a “life-like state” or as little alteration as possible to the living tissue, and to do this in such a way as to allow for the preparation of thin, stained sections.
Detailed explanation-3: -In the fields of histology, pathology, and cell biology, fixation is the preservation of biological tissues from decay due to autolysis or putrefaction. It terminates any ongoing biochemical reactions and may also increase the treated tissues’ mechanical strength or stability.
Detailed explanation-4: -The Aim of Histology Fixation The aim of fixation is to preserve cells or tissues in as near a life-like condition as possible, prevent autolysis and putrefaction, and protect the tissue from damage during subsequent processing.