MICROANATOMY

Detailed explanation-1: -R = 0.203 nm. In practise the resolving power is less than this, mainly because it is impossible to make electromagnetic lenses to the same degree of perfection as glass lenses. Usually with biological specimens, the limit of resolution is around 1-2nm, and the greatest magnification is around 50, 000.

Detailed explanation-2: -A compound light microscope has a maximum resolution of 0.2 µm, this means it can distinguish between two points ≥ 0.2 µm, any objects closer than 0.2um will be seen as 1 object.

Detailed explanation-3: -More common dedicated high-resolution TEMs have a spatial resolution of 0.17 nm, while general-purpose microscopes have spatial resolutions of slightly more than 0.2 nm.

Detailed explanation-4: -The resolution of a modern TEM is about 0.2 nm. This is the typical separation between two atoms in a solid. This resolution is 1, 000 times greater than a light microscope and about 500, 000 times greater than that of a human eye.