INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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The 100X lens should be used in conjunction with a 15X eyepiece only
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The 100X lens should only be used with low light intensity
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100X lenses should be used with a few drops of immersion oil to enhance the image
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When switching to the 100X lens, a wet cloth should be used to clean the lens for clarity
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Detailed explanation-1: -Answer and Explanation: Immersion oil is used for viewing specimens with 100x objectives to help create a pathway for light. Light travels differently through air versus glass. As you increase the magnification, this distortion becomes more noticeable.
Detailed explanation-2: -Place a drop of immersion oil on the top of your cover slip and another drop directly on your 100x oil objective lens. Slowly rotate your 100x oil objective lens into place and adjust the fine focus until you get a crisp and clear image.
Detailed explanation-3: -By placing immersion oil between the glass slide and the oil immersion lens (100X), the light rays at the highest magnification can be retained. Immersion oil has the same refractive index as glass so the oil becomes part of the optics of the microscope.