INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which is correct about dark field
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Background is bright and microbial cells are dark
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Background is dark and microbial cells are bright
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Background is dark
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Background is bright and microbial cells appear stained
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Explanation:
Detailed explanation-1: -In optical microscopes a darkfield condenser lens must be used, which directs a cone of light away from the objective lens. To maximize the scattered light-gathering power of the objective lens, oil immersion is used and the numerical aperture (NA) of the objective lens must be less than 1.0.
Detailed explanation-2: -It is commonly used to observe spirochetes It converts refractivelandex into sample contrast. is a type of electron microscopy It is a type of fluorescence microscopy It can resolve objects less than 1 micrometer.
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