MICROANATOMY

Detailed explanation-1: -The lasers commonly employed in laser scanning confocal microscopy are high-intensity monochromatic light sources, which are useful as tools for a variety of techniques including optical trapping, lifetime imaging studies, photobleaching recovery, and total internal reflection fluorescence (TIRF).

Detailed explanation-2: -Lasers are used in confocal microscopes because they provide: 1) Single wavelength (very pure color) light and 2) very bright light. These usually non-pulsed gas lasers.

Detailed explanation-3: -The availability of UV light for confocal microscopy enables studies of naturally occurring fluorophores. The potential applications of the microscope are illustrated by the real-time imaging of hormone traffic using the naturally occurring oestrogen coumestrol.