INTRODUCTION TO MICROSCOPY CELL BIOLOGY
MICROSCOPY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Negative stain
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Endospore stain
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Gram stain
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Acid Fast stain
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Detailed explanation-1: -Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria.
Detailed explanation-2: -The most commonly used acid-fast stains for visualizing mycobacteria are the Ziehl–Neelsen (ZN) stain and, more recently, the auramine-rhodamine stain.
Detailed explanation-3: -The primary stain used in acid-fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat in the form of heat (steam).
Detailed explanation-4: -Two types of stains are used specifically for detection of mycobacteria: fluorochrome (recommended) and carbol fuchsin.