MICROANATOMY

Detailed explanation-1: -Dark field and phase contrast microscopes allow to observe transparent samples. The dark field microscope produces a light cone, which reaches the objective only when it is scattered by the sample. This reinforces the image contrast.

Detailed explanation-2: -Dark-field microscopy is ideally used to illuminate unstained samples causing them to appear brightly lit against a dark background. This type of microscope contains a special condenser that scatters light and causes it to reflect off the specimen at an angle.

Detailed explanation-3: -Types of Specimens for Dark Field If there are too many items or if a thick solid specimen emits light into the microscope, there will be no dark field. Very thin histological sections can be utilised if they are unstained or very partially stained, such as with silver stains.