MICROANATOMY

Detailed explanation-1: -snRNA-seq, also known as single nucleus RNA sequencing, single nuclei RNA sequencing or sNuc-seq, is an RNA sequencing method for profiling gene expression in cells which are difficult to isolate, such as those from tissues that are archived or which are hard to be dissociated.

Detailed explanation-2: -Smooth muscle cells are spindle shaped, have a single, centrally located nucleus, and lack striations. They are called involuntary muscles. Cardiac muscle has branching fibers, one nucleus per cell, striations, and intercalated disks.

Detailed explanation-3: -A zygote contains a single nucleus. A zygote is formed by the fusion of the sperm and ovum cells by the fertilization process, and a single uni-nucleus is formed in the zygote.

Detailed explanation-4: -snRNA-Seq uses a mild and quick nuclear dissociation protocol to isolate and sequence RNA within the nucleus. The method minimizes technical issues that can arise from common dissociation protocols, especially in studying immediate early gene (IEG) behavior.