NURSING EXAM QUESTIONS
BIOCHEMISTRY
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Question
[CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
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Which are the denaturising factors?
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Elevated temperature, organic solvents, acids, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
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Elevated temperature, acids, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
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Elevated temperature, organic solvents, alkalis, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
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Elevated temperature, organic solvents, acids, heavy metal ions, concentrated solutions of urea or guanidine hydrochloride
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Explanation:
Detailed explanation-1: -A wide variety of reagents and conditions, such as heat, organic compounds, pH changes, and heavy metal ions can cause protein denaturation.
Detailed explanation-2: -Denaturation refers to the physical changes that take place in a protein exposed to abnormal conditions in the environment. Heat, acid, high salt concentrations, alcohol, and mechanical agitation can cause proteins to denature.
Detailed explanation-3: -Physical agents: Heat, surface action, ultraviolet light, ultrasound, high pressure etc. ADVERTISEMENTS: Chemical agents: Acids, alkalis, heavy metal salts, urea, ethanol, guanidine detergents etc. Urea and guanidine probably interfere with the hydrogen bonds between peptide linkages.
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