NURSING ANM AND GNM

NURSING EXAM QUESTIONS

MICROBIOLOGY

Question [CLICK ON ANY CHOICE TO KNOW THE RIGHT ANSWER]
In isopycnic centrifugation only particle size only affects the rate at which particles move until their density is the same as the surrounding gradient medium
A
True
B
False
C
Either A or B
D
None of the above
Explanation: 

Detailed explanation-1: -In isopycnic separation, also called buoyant or equilibrium separation, particles are separated solely on the basis of their density. Particle size only affects the rate at which particles move until their density is the same as the surrounding gradient medium.

Detailed explanation-2: -Principles of Centrifugation Also, the sedimentation rate is zero when the density of the particle is equal to the density of the surrounding medium. Further, the sedimentation rate decreases with increasing viscosity of the medium and increases with increasing centrifugal force applied.

Detailed explanation-3: -Definition: A method where the components of a sample are separated on the basis of their density, in a dense medium or density gradient, in a centrifuge, according to the centrifugal force they experience.

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